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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
20/02/2013 |
Data da última atualização: |
20/04/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, V. de; FILHO, A. N.; MELO, P. de C.; FERNANDES, G. M.; FERRAUDO, A. S.; CONDE, S. de O.; FOGAÇA JÚNIOR, F. A. |
Afiliação: |
VIVIANE DE SOUZA, CNPC; Antonio Nader Filho, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP; Poliana de Castro Melo, UNESP - Jaboticabal, SP; Guilherme Moraes Ferraudo, UNESP - Jaboticabal, SP.; Antônio Sérgio Ferraudo, UNESP - Jaboticabal, SP; Sandra de Oliveira Conde, UNESP - Jaboticabal, SP; Flavio Augusto Fogaça Junior, UNESP - Jaboticabal, SP. |
Título: |
Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012. |
Idioma: |
Português |
Conteúdo: |
Abstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated. MenosAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The ab... Mostrar Tudo |
Palavras-Chave: |
Brasil; Bulk tank; Minas Gerais; PCR; PFGE. |
Thesagro: |
Doença animal; Mamite; Staphylococcus aureus; Vaca leiteira. |
Thesaurus Nal: |
Brazil; Dairy cows; Mammary gland diseases; Mastitis. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76787/1/API-Molecular-epidemiology.pdf.pdf
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Marc: |
LEADER 02617naa a2200349 a 4500 001 1950132 005 2015-04-20 008 2012 bl uuuu u00u1 u #d 100 1 $aSOUZA, V. de 245 $aMolecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.$h[electronic resource] 260 $c2012 520 $aAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated. 650 $aBrazil 650 $aDairy cows 650 $aMammary gland diseases 650 $aMastitis 650 $aDoença animal 650 $aMamite 650 $aStaphylococcus aureus 650 $aVaca leiteira 653 $aBrasil 653 $aBulk tank 653 $aMinas Gerais 653 $aPCR 653 $aPFGE 700 1 $aFILHO, A. N. 700 1 $aMELO, P. de C. 700 1 $aFERNANDES, G. M. 700 1 $aFERRAUDO, A. S. 700 1 $aCONDE, S. de O. 700 1 $aFOGAÇA JÚNIOR, F. A. 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1646-1650, out./dez. 2012.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registros recuperados : 5 | |
1. | | SOUZA, V. de; NADER FILHO, A.; CONDE, S.; MELO, P.; FOGAÇA JÚNIOR, F. A. Identification of enterotoxigenic genes of Staphylococcus aureus strains isolates from artisanal Minas cheese. In: CONGRESO INTERNACIONAL DE ZOONOSIS Y ENFERMIDADES EMERGENTES, 1.; CONGRESO ARGENTINO DE ZOONOSIS, 7., 2011, Bueno Aires. Programa y libro de resúmenes. Buenos Aires: Asociación Argentina de Zoonosis, 2011. p. 178. Resumo V2-28845.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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2. | | FOGAÇA JÚNIOR, F. A.; SOUZA, V. de; NADER FILHO, A.; CONDE, S. de O.; MELO, P. de C. Sensibilidade antimicrobiana de estirpes de Staphylococcus aureus isoladas no leite de vacas com mastite subclínica. Higiene Alimentar, v. 25, n. 194/195, p. 966-968, mar./abr. 2011. Edição dos Trabalhos apresentados no V Congresso Latino Americano e XI Congresso Brasileiro de Higienistas de Alimentos, I Encontro Nacional de Vigilância de Zoonoses e Agravos Causados por Animais de Interesse à Saúde Pública, III...Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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3. | | SOUZA, V. de; MELO, P. de C.; NADER FILHO, A.; CONDE, S. de O.; MEDEIROS, M. I. M. de; FOGAÇA JÚNIOR, F. A. Caracterização de estirpes de Staphylococcus aureus isoladas de queijo Minas artesanal. Higiene Alimentar, v. 25, n. 194/195, p. 894-896, mar./abr. 2011. Edição dos Trabalhos apresentados no V Congresso Latino Americano e XI Congresso Brasileiro de Higienistas de Alimentos, I Encontro Nacional de Vigilância de Zoonoses e Agravos Causados por Animais de Interesse à Saúde Pública, III...Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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4. | | SOUZA, V. de; NADER FILHO, A.; MELO, P. de C.; FERRAUDO, G. M.; FERRAUDO, A. S.; CONDE, A. de O.; FOGAÇA JÚNIOR, F. A. Epidemiologia molecular dos Staphylococcus aureus isolados em diferentes pontos do fluxograma de produção do leite. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011. Resumo: 486-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | | SOUZA, V. de; FILHO, A. N.; MELO, P. de C.; FERNANDES, G. M.; FERRAUDO, A. S.; CONDE, S. de O.; FOGAÇA JÚNIOR, F. A. Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 5 | |
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